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main skeletal muscle mhc isoforms  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank main skeletal muscle mhc isoforms
    Effect of NMES on the response of muscle fiber size to total knee arthroplasty (TKA). Representative immunohistochemistry images for pre-surgery and 5-wk post-surgery Control ( A and B , respectively) and NMES ( C and D , respectively) groups. Tissue sections treated with antibodies recognizing myosin heavy chain <t>(MHC)</t> I (green), MHC IIA (red), and MHC IIX (blue) <t>isoforms</t> are shown. As fibers expressing only MHC IIX are rare, the large majority of MHC IIX expression occurs in MHC IIAX hybrid fibers (purple). Scale bar = 50 um. Skeletal muscle fiber cross-sectional area (CSA) calculated from minimal Feret diameter ( E ) for all fiber types pooled together (All Fibers) and MHC I, IIA, and IIAX fibers are shown at pre-surgery (open bar) and post-surgery (filled bar) for patients in control (black) and NMES (blue) groups. Analyses were limited to fibers expressing MHC I, IIA, and IIAX isoforms because other MHC isotype fibers (MHC I/IIA, IIX, and I/IIA/IIX) were too few to permit analyses. Bars represent mean and SE from parameter estimates from the linear mixed model analysis for n = 10 patients for each time point derived, except for n = 9 patients for MHC IIAX post-surgery because one patient did not have any MHC IIAX fibers at one time point. Data points represent individual volunteer average values for single fibers clustered within each volunteer for baseline and 5-week post-surgery evaluations. Please note that these individual average data are presented for data transparency purposes only and are not utilized in the statistical analytical model. Bar graphs represent least squared means and standard error values derived from the mixed model analysis. Deviation of individual values from bars graph data are due to the fact model-derived values reflect adjustment for the effects of sex, as well as other factors. In addition, many of the measures were log transformed for analysis, and as such the least squared means and standard errors plotted were back transformed into original units. Thus, these values can be somewhat offset from those of the raw data. P values indicate time (T) and group X time (GxT) effects (atop brackets) or within group comparisons over time (over lines). * P < 0.05, *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Main Skeletal Muscle Mhc Isoforms, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/main+skeletal+muscle+mhc+isoforms/anti-laminin/pmc12445151-127-7-21
    Average 94 stars, based on 73 article reviews
    main skeletal muscle mhc isoforms - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Sustaining neuromuscular activation after total knee arthroplasty preserves skeletal muscle fiber size, contractility, and innervation in older adults"

    Article Title: Sustaining neuromuscular activation after total knee arthroplasty preserves skeletal muscle fiber size, contractility, and innervation in older adults

    Journal: Experimental gerontology

    doi: 10.1016/j.exger.2025.112831

    Effect of NMES on the response of muscle fiber size to total knee arthroplasty (TKA). Representative immunohistochemistry images for pre-surgery and 5-wk post-surgery Control ( A and B , respectively) and NMES ( C and D , respectively) groups. Tissue sections treated with antibodies recognizing myosin heavy chain (MHC) I (green), MHC IIA (red), and MHC IIX (blue) isoforms are shown. As fibers expressing only MHC IIX are rare, the large majority of MHC IIX expression occurs in MHC IIAX hybrid fibers (purple). Scale bar = 50 um. Skeletal muscle fiber cross-sectional area (CSA) calculated from minimal Feret diameter ( E ) for all fiber types pooled together (All Fibers) and MHC I, IIA, and IIAX fibers are shown at pre-surgery (open bar) and post-surgery (filled bar) for patients in control (black) and NMES (blue) groups. Analyses were limited to fibers expressing MHC I, IIA, and IIAX isoforms because other MHC isotype fibers (MHC I/IIA, IIX, and I/IIA/IIX) were too few to permit analyses. Bars represent mean and SE from parameter estimates from the linear mixed model analysis for n = 10 patients for each time point derived, except for n = 9 patients for MHC IIAX post-surgery because one patient did not have any MHC IIAX fibers at one time point. Data points represent individual volunteer average values for single fibers clustered within each volunteer for baseline and 5-week post-surgery evaluations. Please note that these individual average data are presented for data transparency purposes only and are not utilized in the statistical analytical model. Bar graphs represent least squared means and standard error values derived from the mixed model analysis. Deviation of individual values from bars graph data are due to the fact model-derived values reflect adjustment for the effects of sex, as well as other factors. In addition, many of the measures were log transformed for analysis, and as such the least squared means and standard errors plotted were back transformed into original units. Thus, these values can be somewhat offset from those of the raw data. P values indicate time (T) and group X time (GxT) effects (atop brackets) or within group comparisons over time (over lines). * P < 0.05, *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Effect of NMES on the response of muscle fiber size to total knee arthroplasty (TKA). Representative immunohistochemistry images for pre-surgery and 5-wk post-surgery Control ( A and B , respectively) and NMES ( C and D , respectively) groups. Tissue sections treated with antibodies recognizing myosin heavy chain (MHC) I (green), MHC IIA (red), and MHC IIX (blue) isoforms are shown. As fibers expressing only MHC IIX are rare, the large majority of MHC IIX expression occurs in MHC IIAX hybrid fibers (purple). Scale bar = 50 um. Skeletal muscle fiber cross-sectional area (CSA) calculated from minimal Feret diameter ( E ) for all fiber types pooled together (All Fibers) and MHC I, IIA, and IIAX fibers are shown at pre-surgery (open bar) and post-surgery (filled bar) for patients in control (black) and NMES (blue) groups. Analyses were limited to fibers expressing MHC I, IIA, and IIAX isoforms because other MHC isotype fibers (MHC I/IIA, IIX, and I/IIA/IIX) were too few to permit analyses. Bars represent mean and SE from parameter estimates from the linear mixed model analysis for n = 10 patients for each time point derived, except for n = 9 patients for MHC IIAX post-surgery because one patient did not have any MHC IIAX fibers at one time point. Data points represent individual volunteer average values for single fibers clustered within each volunteer for baseline and 5-week post-surgery evaluations. Please note that these individual average data are presented for data transparency purposes only and are not utilized in the statistical analytical model. Bar graphs represent least squared means and standard error values derived from the mixed model analysis. Deviation of individual values from bars graph data are due to the fact model-derived values reflect adjustment for the effects of sex, as well as other factors. In addition, many of the measures were log transformed for analysis, and as such the least squared means and standard errors plotted were back transformed into original units. Thus, these values can be somewhat offset from those of the raw data. P values indicate time (T) and group X time (GxT) effects (atop brackets) or within group comparisons over time (over lines). * P < 0.05, *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Immunohistochemistry, Control, Expressing, Derivative Assay, Transformation Assay

    Related Articles

    Immunopeptidomics:

    Article Title: Sustaining neuromuscular activation after total knee arthroplasty preserves skeletal muscle fiber size, contractility, and innervation in older adults
    Article Snippet: .. Briefly, primary antibodies raised against the three main skeletal muscle MHC isoforms (MHC I, IIA, IIX; BA-D5, SC-71, and 6H-1 from Developmental Studies Hybridoma Bank, Iowa City, IA) are used to identify all 6 fiber isotypes (MHC I, I/IIA, IIA, IIA/X, IIX, I/IIA/IIX). .. Image acquisition was performed with an Olympus BX51 microscope (Olympus America, Center Valley, PA) at ×20, with analysis using ImageJ.

    Article Title: Sustaining neuromuscular activation after total knee arthroplasty preserves skeletal muscle fiber size, contractility, and innervation in older adults.
    Article Snippet: .. Briefly, primary antibodies raised against the three main skeletal muscle MHC isoforms (MHC I, IIA, IIX; BA-D5, SC-71, and 6H-1 from Developmental Studies Hybridoma Bank, Iowa City, IA) are used to identify all 6 fiber isotypes (MHC I, I/IIA, IIA, IIA/X, IIX, I/IIA/IIX). .. Image acquisition was performed with an Olympus BX51 microscope (Olympus America, Center Valley, PA) at ×20, with analysis using ImageJ.



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    main skeletal muscle mhc isoforms  (Developmental Studies Hybridoma Bank)
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    Developmental Studies Hybridoma Bank main skeletal muscle mhc isoforms
    Effect of NMES on the response of muscle fiber size to total knee arthroplasty (TKA). Representative immunohistochemistry images for pre-surgery and 5-wk post-surgery Control ( A and B , respectively) and NMES ( C and D , respectively) groups. Tissue sections treated with antibodies recognizing myosin heavy chain <t>(MHC)</t> I (green), MHC IIA (red), and MHC IIX (blue) <t>isoforms</t> are shown. As fibers expressing only MHC IIX are rare, the large majority of MHC IIX expression occurs in MHC IIAX hybrid fibers (purple). Scale bar = 50 um. Skeletal muscle fiber cross-sectional area (CSA) calculated from minimal Feret diameter ( E ) for all fiber types pooled together (All Fibers) and MHC I, IIA, and IIAX fibers are shown at pre-surgery (open bar) and post-surgery (filled bar) for patients in control (black) and NMES (blue) groups. Analyses were limited to fibers expressing MHC I, IIA, and IIAX isoforms because other MHC isotype fibers (MHC I/IIA, IIX, and I/IIA/IIX) were too few to permit analyses. Bars represent mean and SE from parameter estimates from the linear mixed model analysis for n = 10 patients for each time point derived, except for n = 9 patients for MHC IIAX post-surgery because one patient did not have any MHC IIAX fibers at one time point. Data points represent individual volunteer average values for single fibers clustered within each volunteer for baseline and 5-week post-surgery evaluations. Please note that these individual average data are presented for data transparency purposes only and are not utilized in the statistical analytical model. Bar graphs represent least squared means and standard error values derived from the mixed model analysis. Deviation of individual values from bars graph data are due to the fact model-derived values reflect adjustment for the effects of sex, as well as other factors. In addition, many of the measures were log transformed for analysis, and as such the least squared means and standard errors plotted were back transformed into original units. Thus, these values can be somewhat offset from those of the raw data. P values indicate time (T) and group X time (GxT) effects (atop brackets) or within group comparisons over time (over lines). * P < 0.05, *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Main Skeletal Muscle Mhc Isoforms, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/main+skeletal+muscle+mhc+isoforms/anti-laminin/pmc12445151-127-7-21
    Average 94 stars, based on 1 article reviews
    main skeletal muscle mhc isoforms - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Effect of NMES on the response of muscle fiber size to total knee arthroplasty (TKA). Representative immunohistochemistry images for pre-surgery and 5-wk post-surgery Control ( A and B , respectively) and NMES ( C and D , respectively) groups. Tissue sections treated with antibodies recognizing myosin heavy chain (MHC) I (green), MHC IIA (red), and MHC IIX (blue) isoforms are shown. As fibers expressing only MHC IIX are rare, the large majority of MHC IIX expression occurs in MHC IIAX hybrid fibers (purple). Scale bar = 50 um. Skeletal muscle fiber cross-sectional area (CSA) calculated from minimal Feret diameter ( E ) for all fiber types pooled together (All Fibers) and MHC I, IIA, and IIAX fibers are shown at pre-surgery (open bar) and post-surgery (filled bar) for patients in control (black) and NMES (blue) groups. Analyses were limited to fibers expressing MHC I, IIA, and IIAX isoforms because other MHC isotype fibers (MHC I/IIA, IIX, and I/IIA/IIX) were too few to permit analyses. Bars represent mean and SE from parameter estimates from the linear mixed model analysis for n = 10 patients for each time point derived, except for n = 9 patients for MHC IIAX post-surgery because one patient did not have any MHC IIAX fibers at one time point. Data points represent individual volunteer average values for single fibers clustered within each volunteer for baseline and 5-week post-surgery evaluations. Please note that these individual average data are presented for data transparency purposes only and are not utilized in the statistical analytical model. Bar graphs represent least squared means and standard error values derived from the mixed model analysis. Deviation of individual values from bars graph data are due to the fact model-derived values reflect adjustment for the effects of sex, as well as other factors. In addition, many of the measures were log transformed for analysis, and as such the least squared means and standard errors plotted were back transformed into original units. Thus, these values can be somewhat offset from those of the raw data. P values indicate time (T) and group X time (GxT) effects (atop brackets) or within group comparisons over time (over lines). * P < 0.05, *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Experimental gerontology

    Article Title: Sustaining neuromuscular activation after total knee arthroplasty preserves skeletal muscle fiber size, contractility, and innervation in older adults

    doi: 10.1016/j.exger.2025.112831

    Figure Lengend Snippet: Effect of NMES on the response of muscle fiber size to total knee arthroplasty (TKA). Representative immunohistochemistry images for pre-surgery and 5-wk post-surgery Control ( A and B , respectively) and NMES ( C and D , respectively) groups. Tissue sections treated with antibodies recognizing myosin heavy chain (MHC) I (green), MHC IIA (red), and MHC IIX (blue) isoforms are shown. As fibers expressing only MHC IIX are rare, the large majority of MHC IIX expression occurs in MHC IIAX hybrid fibers (purple). Scale bar = 50 um. Skeletal muscle fiber cross-sectional area (CSA) calculated from minimal Feret diameter ( E ) for all fiber types pooled together (All Fibers) and MHC I, IIA, and IIAX fibers are shown at pre-surgery (open bar) and post-surgery (filled bar) for patients in control (black) and NMES (blue) groups. Analyses were limited to fibers expressing MHC I, IIA, and IIAX isoforms because other MHC isotype fibers (MHC I/IIA, IIX, and I/IIA/IIX) were too few to permit analyses. Bars represent mean and SE from parameter estimates from the linear mixed model analysis for n = 10 patients for each time point derived, except for n = 9 patients for MHC IIAX post-surgery because one patient did not have any MHC IIAX fibers at one time point. Data points represent individual volunteer average values for single fibers clustered within each volunteer for baseline and 5-week post-surgery evaluations. Please note that these individual average data are presented for data transparency purposes only and are not utilized in the statistical analytical model. Bar graphs represent least squared means and standard error values derived from the mixed model analysis. Deviation of individual values from bars graph data are due to the fact model-derived values reflect adjustment for the effects of sex, as well as other factors. In addition, many of the measures were log transformed for analysis, and as such the least squared means and standard errors plotted were back transformed into original units. Thus, these values can be somewhat offset from those of the raw data. P values indicate time (T) and group X time (GxT) effects (atop brackets) or within group comparisons over time (over lines). * P < 0.05, *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Briefly, primary antibodies raised against the three main skeletal muscle MHC isoforms (MHC I, IIA, IIX; BA-D5, SC-71, and 6H-1 from Developmental Studies Hybridoma Bank, Iowa City, IA) are used to identify all 6 fiber isotypes (MHC I, I/IIA, IIA, IIA/X, IIX, I/IIA/IIX).

    Techniques: Immunohistochemistry, Control, Expressing, Derivative Assay, Transformation Assay